FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2472.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2472.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1511045
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28310.18735378496338626No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC5550.032.646046145
GCTGCCC5050.030.137796145
CTGCCGC13550.028.882715145
GTATAAG6950.025.0386681
GTCCGGT1902.1922888E-422.8972031
CCCCTAG18100.022.4333571
TCTGACC9650.021.02879145
CTGTACG8450.020.5871189
CCTAGAC4952.1827873E-1120.5011523
CCTAGTG16150.020.1973973
CCCTAGT18450.019.645262
TACTCCG4553.448804E-919.1159785
TGTAAGG5356.548362E-1118.9696122
TATTAGG7000.018.6405452
CTTAACT11400.018.4388664
CCCTAGA4354.2808097E-818.331062
GTTAAGG10050.018.036771
GTATTAG6052.0008883E-1117.9771441
AGAGTGC10500.017.947788
TATCCCG3352.8660883E-517.3089945
Produced by FastQC (version 0.11.2)