FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n02_qg2471.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n02_qg2471.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1201884
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19890.1654901804167457No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC4700.050.894962145
GCTGCCC5200.046.001217145
GTATAAG6000.033.8384251
CTGCCGC10900.029.925732145
TCTGACC8600.025.286081145
GTAGGAC1751.2560685E-424.8567453
CCTAGTG7500.024.1662773
CCCCTAG8000.023.5660441
CCCTAAG1851.8290998E-423.517051
CCCTAGT8950.022.6821442
TATACTA5500.021.0888165
CTTAGGC14100.020.5670453
GCCGTAA3208.488478E-720.386904145
TATAAGA12050.019.8552782
GCTTAGG13900.019.820652
ATAGCCC2205.904954E-419.7724083
GTATATA9550.019.7411881
GTGTATA7600.019.0818181
GTACTGG4603.927198E-918.915891
GGCTTAG14750.018.6807751
Produced by FastQC (version 0.11.2)