FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2600.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2600.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3331385
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC15000.043.983604145
CGCTACC10550.033.67319145
CGCTATG21700.029.401102145
GTATAAG16400.026.5357061
CTGCTTT40850.025.912033145
TGCCGTG39900.025.257046145
GTGTATA17250.022.7053341
CCACGAC17250.021.434914145
CTCCGAC20600.020.76469145
GTATTAT19700.020.6179281
CGAGACC27450.020.337101145
TATACCG7850.020.3243945
CGTCTTG57550.019.27466145
GTCCTAG9800.019.2428991
ACACATA33950.019.219557145
ATCTCCC19650.018.447948145
TATAAGA27850.017.9680712
GTTATAG10350.017.5195471
TGTATAA22800.017.4946562
GTATAGA12100.017.3834461
Produced by FastQC (version 0.11.2)