FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2599.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2599.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5391479
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC23700.029.673712145
TGCCGTG34250.029.635752145
CCACGAC24550.022.739859145
GTATAGG13750.022.6789551
CTGCTTT56100.021.582518145
CTCCGAC30100.020.714787145
GTATAAG23500.020.0586991
CGTCTTG55150.019.719425145
GTATATA35950.018.9620911
TTATACT46000.018.7584974
CCTAGTG31800.018.6978473
CAGGCAC17700.018.432648145
CTGTACG21100.018.2106679
ATAATAC44650.018.0262933
GTATTAA26100.016.9490971
GTATAAA46300.016.9160921
CCCCTAG36900.016.7051281
GTATTAG18800.016.5870021
AGGGGTA48450.016.3134445
TAAGGGG44550.016.2763563
Produced by FastQC (version 0.11.2)