FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2598.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2598.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2031902
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG7500.035.766163145
GCCCACC9750.032.717487145
TAGGACA18300.024.5691474
ACAGTGC9100.023.104648
GTCCTAG16200.022.8304961
GTATATA12750.022.182711
TACACAG10550.021.9961645
CTGTACG12200.021.393679
CTCTAGG20700.021.3707451
CTGCTTT20800.021.26172145
CTAGGAC18800.021.2155513
CCACGAC10300.021.116207145
GTACTAC5251.8189894E-1220.7201121
TAATACG4201.2660166E-920.7196034
TACACCG3651.358203E-719.8681135
CCTAGAG18750.019.7250613
GTATAGA9200.019.706631
GTGCTAA4102.1629603E-819.456691
AGGACAC22450.019.3813675
CCCACGC10600.019.150673145
Produced by FastQC (version 0.11.2)