FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2597.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2597.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2074486
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG7300.033.766697145
GTGTATA12600.027.0511341
GTATAAG12500.024.9469011
GCCCACC9050.024.032846145
GGCCGTA2505.979043E-520.2997447
GTCCTAG12750.019.9074671
TGCACCG4452.6011548E-919.55555
GTATATA14350.019.2039181
CCCACGC7950.019.150702145
TACACAG9500.019.0837655
CGTCTTG16100.018.012196145
TATAAGA19150.017.798232
CCCTAGT31250.017.4043962
CCCCTAG30450.017.3857611
CCTAGTG29800.017.2766043
GTATAGG6354.1836756E-1117.1307241
CCTAGAG14950.016.9763033
TAGTGAC34700.016.9279065
CTACATG12900.016.860252145
GAGCCCC13100.016.602842145
Produced by FastQC (version 0.11.2)