FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2595.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2595.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3635945
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG21250.033.446841
GCCCACC18500.032.52752145
GTGTATA20500.029.0100151
TATAAGA30850.026.5625292
CTGCTTT50850.022.66996145
TATACCG7400.022.5393945
GTCTAAG4058.039933E-1021.4889011
TAAGAGA41400.021.194894
TGTATAA24850.021.0112692
CCACGAC18300.020.997587145
ATAAGAG38800.020.9330463
CAGATGT28450.020.391724
GTATAAC8750.019.0637251
AGATGTG23900.018.5087915
GAGACAG33900.018.3915987
CTAAGAC7900.018.3590013
ACTTAGC5158.076313E-1018.299998
ACCGTCC6750.018.258288
CGTCAGA19500.018.2242411
TAGGACA9600.018.1295134
Produced by FastQC (version 0.11.2)