FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2594.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2594.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133112
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT3910.29373760442334274No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC3790.2847226395817056No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG3260.244906544864475No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA1990.14949816695714885No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA1780.1337219784842839No Hit
AATAAAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGA1430.10742833102950898No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTTACG100.0070843915144.997361
ATTACGC450.00962161648.332463
AGGACAG450.00962161648.332465
CGTTATG1905.353286E-934.341481
CCCTAGA850.00273373734.1170271
CGACCTC850.00273373734.1170276
TAACGTT1707.737435E-834.1170275
CCTGTGA1956.897608E-933.460933
TTCGACC1102.4251343E-432.953954
GGTCAAC900.00361734932.2216381
GCTCTAA900.00361734932.2216381
TCGACCT900.00361734932.2216385
CCCTGTG2051.123226E-831.8286912
ATAGACA950.00471316130.525764
AAAGTAG950.00471316130.525765
AAACCTA950.00471316130.525765
CTATTCG950.00471316130.525761
TGGTGAT950.00471316130.525765
ACGTTTC2701.6916601E-1029.53657
ATAACGT1753.7429036E-628.9994744