FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2593.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2593.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1402296
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG4550.041.43099145
GCCCACC7350.027.620659145
GTATAAG7800.026.9624651
ACAGTGC5600.024.5970178
CTGTACG9500.023.6567449
CCTTAAC11050.022.9692343
CTTAACT13250.022.4392724
CCACGAC7700.021.657106145
TACACTA4851.6370905E-1120.9327895
GTATTAA6200.019.8844451
TATACTG10050.019.4822225
GTATTAT10450.019.4311281
CCTGTAC3401.512275E-619.1957173
CACGAGC7950.019.152061145
CTTATAA10700.018.973068145
GTGTATA8450.018.8808781
GTATAAA12750.018.7698151
GTATATA10050.018.7613281
GTATATG6650.018.5388811
CCTAGTG10600.018.4713523
Produced by FastQC (version 0.11.2)