FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2590.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2590.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040717
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG9900.040.278137145
GCCCACC10550.037.796547145
GTGTATA12550.026.5804561
CCACGAC10750.024.279285145
GTATAAG10200.023.4601153
AGTACCG4053.274181E-1123.2763775
GCAGCGT5002.5465852E-1120.3051571
TGTATAA15250.019.0198272
ACACATA24200.017.975368145
TATAAGA18100.017.2268644
ATCTCCC12700.017.126137145
CGTCTTG17850.017.058977145
TGCACCG4701.0331132E-716.9715655
CACGAGC12250.016.571577145
CTGCTTT21700.016.371113145
GCCATAG4007.1291797E-616.3166451
AGACGGC22250.016.29228145
GTATATG9550.015.9464591
GTACAAG8850.015.5689271
GTATAGC4201.13264705E-515.5396611
Produced by FastQC (version 0.11.2)