FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2589.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2589.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1814684
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8500.030.70662145
GTATAAG12000.028.4019931
TGCCGTG7950.021.887318145
TATAAGA18650.021.3853342
GTATAGA9850.021.3498081
GTATAAA14950.020.372311
CCCACGC7050.019.539476145
CATGATG30650.019.396875145
GTGTATA11400.018.4469811
ACCGTGG3201.9293997E-518.1249358
CTATACT6553.6379788E-1217.7142544
GTCTAAG4205.9364174E-717.265651
TAACGTT13050.017.2259585
CTCCGAC10700.016.939657145
GTATATA13300.016.902161
CTACCCT3905.603917E-616.7348584
AGGCTTA37450.016.4588436
CTGTACG8850.016.384129
GTATTAG7651.8189894E-1216.1146051
GTATAGG5904.8949005E-915.9780411
Produced by FastQC (version 0.11.2)