FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2588.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2588.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2120004
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC58220.2746221233544842No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT44690.21080148905379426No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG41040.19358454040652753No Hit
GGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAA26730.1260846677647778No Hit
GTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAATAGAGGTCAAA23840.11245261801392827No Hit
CTTACACACTTACACACTTACACACTTACACACTTACACACTTACACACT21540.1016035818800342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCT11100.029.4006885
CGACCTC12350.025.8376886
TGTCGGT13300.025.6279682
TCGGTGT14350.023.752754
AGTGACC83250.023.2592096
CCTAGTG76300.023.1917343
CGTTATG17300.023.0571171
CTAGTGA81500.022.9577874
TAGTGAC83100.022.9521125
CCCCTAG81150.022.4322911
CCCTAGT81200.022.4174212
CACAACG15800.022.0319063
CAACGTT16700.021.7130835
CCTGTGA23200.021.5689533
TTCGACC15500.021.5225684
GTCGGTG14850.021.4879113
ACAATGC24750.021.3836788
CAATGCG24500.021.3059649
CGGTGTG16000.021.3032475
GTATAAG9550.020.5044821
Produced by FastQC (version 0.11.2)