FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2587.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2587.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1082234
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTGCC750.001483967638.6638689
ACCCGTG1553.4729965E-837.416657
GTGTATA4550.036.657561
TTATAGT4400.034.6093644
GTCTAAG1051.8510823E-434.5324821
GTATAAT5900.034.415421
CTGCTTT10750.033.048702145
TGGACCG1551.4628113E-632.7486465
GTCCTGC1352.1386875E-532.2303121
CGAGCGT900.003624722432.2288256
GTATAAG4100.031.835793
CTTAGGA1601.8743049E-631.7252522
GTGGACC1153.158471E-431.52824
AGTACCG1402.7430397E-531.0777955
GTGCTAG950.00472166130.5339831
GGCCGTT950.00472913830.5241097
GTACCGT2151.807166E-830.3550576
CTAAGGG1504.392893E-529.0059433
TATTAGG3251.8189894E-1229.0059412
CCGTGCA1255.1563E-428.9979029
Produced by FastQC (version 0.11.2)