FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2585.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2585.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1705591
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC24370.14288302412477552No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG21970.12881165531478533No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7650.028.42984145
TGCCGTG6950.027.120838145
CGTTATG15900.023.7163621
CCCTGTG13800.022.0698492
CCCCTAG31000.021.5212761
CAATGCG20850.021.2098879
GTATAAG8900.021.1848431
CACAACG14600.020.8605423
CCTGTGA14750.020.6484013
CCTAGTG29400.020.2253343
ACAACGT14550.019.9354574
TTATGAC23200.019.6916333
CTGTACG7750.019.64419
CTGTGAC15650.019.4609554
ACAATGC23100.019.4578258
TATGACA24700.019.0829524
CCCTAGT31800.018.9269262
GTCGGTG10950.018.5427063
CAACGTT16050.018.5241375
CTAGTGA38450.018.2938274
Produced by FastQC (version 0.11.2)