FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2584.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2584.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1972587
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT27880.1413372388644962No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC27100.13738304064662293No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG20770.10529320126311285No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG8200.024.7676141
GCCCACC10200.023.457277145
TAACGTT16700.022.580775
CAATGCG21350.022.4112389
CGTTATG14750.022.1289351
ATAACGT15850.021.9615944
CCTGTGA14900.021.9011613
ACAATGC24400.021.6896638
CCTAGTG38250.021.4233763
CACGAGC18000.021.34849145
TGCCGTG6950.020.86455145
CCCTGTG15450.020.6526642
CACAACG13550.020.3369223
GTATATA16350.019.9634111
TAAAGGT34100.019.7774123
CCCCTAG43750.019.7291761
CCCTAGT43400.019.7171542
ACGTTTC32750.019.4800997
CTGTGAC17150.019.0283154
CAACGTT15350.018.8974655
Produced by FastQC (version 0.11.2)