FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2583.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2583.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1240399
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTACC4150.026.20693145
GTATAAG6150.024.761091
TGCCGTG11250.023.846367145
GCCCACC6400.023.790981145
GTATAGA5250.022.0996911
CCTAGTG17900.021.8750363
GTGTATA8400.021.581731
TAGGACA8400.021.580864
CCCCTAG18500.021.166431
CCTTATA4501.3278623E-1020.9478232
CCCTAGT18950.020.6629622
CTAGGAC7450.020.4395373
CTGCTTT13200.019.77432145
CTAGTGA23250.019.336454
CTCTAGG8700.019.170531
GTATTAT8400.018.9919221
CCCTGTG8150.018.6794789
TATACTA4354.2651664E-818.336295
TCTATAC5601.2551027E-1018.1279243
TAGTGAC24950.018.0189385
Produced by FastQC (version 0.11.2)