FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2582.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2582.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences574804
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTATAG900.003621142632.232781
TAAGTAC1358.0671604E-426.8583133
GTCCTAA1658.393731E-526.3722741
GCCCACC2507.820199E-826.099466145
GTAGACT2259.077139E-725.7839814
TATAGGA1751.252029E-424.8652882
TCTGTAC2401.592769E-624.1724823
CCGTTCG2451.9100644E-623.6729879
CTGTACG1851.8296011E-423.5130359
GCAGCGT1550.001804115723.394761
GTAGTAG2852.7657734E-722.9022391
AGGACAG1952.6086177E-422.313065
TAACAGT2653.7674163E-621.892066
GCGTCAG1700.003085344321.3305171
CTATACA3106.2200525E-721.0534524
GTATATG2104.301537E-420.7210731
AGCGTCA1750.003651769620.719273
GAACCGT2505.957797E-520.3048866
CAGCGTC1800.004296723320.1454892
GCCGTTC1800.00430538920.1384778
Produced by FastQC (version 0.11.2)