FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2581.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2581.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2168378
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10500.034.523937145
TGCCGTG8650.025.144602145
GTGTATA15000.024.6586191
GTATAAG13200.023.6256831
TACCCCA7800.023.243165
AGACCGC11300.023.09743145
GTATTAA10550.019.9358791
GTATATG11900.019.5026151
CCCACGC9200.018.913113145
GAGACCC14650.018.805529145
TTAGTAC7250.018.0046333
CTCCGAC12500.017.980066145
CCACGAC11850.017.742682145
CGTCTTG16000.017.67194145
CTGTACG13200.017.5750129
CCCCTAG33050.017.5553041
CCTAGTG31250.016.70833
GTATATA16500.016.7028081
TATACTT18400.016.5531735
CTTAGGC29550.016.442473
Produced by FastQC (version 0.11.2)