FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2580.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2580.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1681500
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC9900.029.293205145
TACACTG8400.027.6242375
TAGGACA12750.026.730514
TACACCG3201.2405508E-924.9265585
CTAGGAC12600.024.1712073
TACACAG7200.024.1712075
CTGCTTT19950.022.531542145
GTATAAG6250.022.048731
CCACGAC8800.021.420658145
TAAGGCC3757.772542E-921.266869145
ATAGGAC4501.3278623E-1020.948383
GTATAGA6950.020.8715741
TATACAG8400.020.7181785
CTCTAGG14500.020.5081211
ACACAGT8600.020.2363596
CCTAGAG14150.019.9860863
GTCCTAG12350.019.967421
TACTAGC3301.1359025E-619.7782062
CATACCC6750.019.3369663
AGGACAC15800.019.2757725
Produced by FastQC (version 0.11.2)