FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2579.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2579.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1435413
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15910.1108391800826661No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8050.029.721174145
GTATAAG7450.029.2075061
TGCCGTG7200.022.153282145
CTGTACG7600.021.9405259
AGACGGC17350.021.311726145
GTGTATA9600.021.1551591
TAGGACA9700.020.9341474
ACCTTTA11350.020.4402838
GTCCTAG10350.020.322971
TATAAGA13750.020.0424062
CTCCGAC8400.019.851643145
CTATACA6250.019.7259484
CTCTAGG10950.019.2093831
GTATATA9900.019.0488011
CCTTAAC7300.018.8755533
GTCCTAA3101.4619374E-518.717931
GTAATAC6251.8189894E-1218.5655983
CCACGAC8300.018.343792145
CTAGGAC9900.018.3136023
CCTAGTG23800.018.2828243
Produced by FastQC (version 0.11.2)