FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2578.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2578.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1561426
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7800.025.09524145
CTATACA8000.023.5714534
GTGTATA9550.022.0240731
TGCCGTG7100.021.442879145
CCACGAC7750.020.579893145
GTATAAG8800.019.7802431
TTATACT16750.019.0520114
GTATTAA7550.018.2519661
TAATACT11000.017.8022174
GTATATA10950.017.2211531
GCAGCGT3804.369813E-617.1775781
GTATTAC5952.9649527E-1017.0653061
CTAAAGG4701.0305848E-716.9745353
TACACCG3854.949079E-616.9544935
TAGGCCC4307.579347E-716.8668734
GCCTTAG2600.001807747516.7371271
CCCCTAG13950.016.6371441
TACACAG7151.4551915E-1116.2299425
TATACTT16100.016.217345
GTACTAT5904.8821676E-915.9806471
Produced by FastQC (version 0.11.2)