FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2577.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2577.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1917697
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20150.10507395068146846No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19600.10220592721373607No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC11100.029.393568145
GTATAAG11300.025.6705361
GTGTATA11900.025.5950321
CCACGAC9600.024.168043145
GTCCTAG7200.020.144241
GTATATA12950.019.5998021
CCCACGC7150.019.266832145
CCCCTAG26800.019.2121931
CCTAGTG24900.018.3453753
TATAAGA18700.018.2258152
TGTATAA15950.018.18572
ACCCTGC2801.4271779E-418.1246158
CTCCGAC11350.017.886482145
TAGGGCA2901.8649406E-417.501914
CTATACT7550.017.2866554
GAGCCCC10750.016.861427145
CCCTTAG3905.6031204E-616.7352141
GTATATG9550.016.7060071
ACACATA23350.016.45704145
TCTAGAC20750.016.423483
Produced by FastQC (version 0.11.2)