FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2576.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2576.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145694
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT1500.10295550949249797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGCGG350.003560591362.14197145
GACTGGG400.006040893554.3742267
TACCCTT450.00961022748.3492435
CTACCCT450.00961022748.3492434
ATACGGA907.4365E-540.2910356
TATACGG750.001476832338.6793945
TACATGT951.0246571E-438.157357
GTGTAGA1001.3829388E-436.2619321
TTTATAC1251.25486185E-534.8114553
GGGGTAC1051.8424005E-434.5351756
TTAATAC900.003612310432.2328263
AAGATTA1153.1428732E-431.5321166
CTAGACT950.004706599330.5363644
TATGACT950.004706599330.5363644
ATCTCCC950.004714515530.525879145
GCCCACC950.004714515530.525879145
AAGAGTC950.004714515530.5258797
CTCCGAC950.004714515530.525879145
GTATTAA1000.006047921729.0095461
TAATACT1050.00767485527.6281414
Produced by FastQC (version 0.11.2)