FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2575.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2575.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1888449
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC19100.10114120105970562No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA12600.025.9004861
CTGTACG8450.025.7390489
GCCCACC9100.025.493916145
GTATAAG10300.021.8268181
GTCCTAG9700.020.9340021
GTATAGA8350.020.8444611
GTAGGAC3851.0504664E-820.7181953
CCCCTTA17400.018.7555261
GTAGCAC4303.738205E-818.550013
CTATACT6700.018.3989934
CTTAACT14200.018.3837494
GCGTCAG5301.1768861E-917.788261
CCTAGAG12000.017.524143
CGTCAGA12850.017.4954261
ACTGTCC7100.017.3587518
CACGAGC15300.017.058428145
CCCCTAG20000.017.0425191
TGTATAA14500.017.0036582
GTACTAC6053.783498E-1016.7818031
GTATATG9950.016.7637311
Produced by FastQC (version 0.11.2)