FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2574.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2574.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865181
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC22230.1191841435228002No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG19580.10497640711544884No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG10600.026.6834281
AGACCGC9000.024.97185145
GCCCACC8250.024.605694145
CCACGAC9200.024.428982145
TGCCGTG7500.024.166306145
TAGGACA13400.021.6455294
GTGTATA10100.020.8237761
TATAAGG6700.020.5643562
GTGTAAC6400.020.3974291
TAATACT11400.020.3543934
TATAAGA16350.019.0715942
ACAGTGC6700.018.3947548
GTAATAC7100.018.3834553
CACGAGC13250.018.056334145
GAGACCC11100.016.981728145
GTATAGA8150.016.907481
CTCCGAC11700.016.730518145
GTCCTAG10850.016.7106421
CTAGGAC13300.016.3562073
GGACCGC4007.134029E-616.3153176
Produced by FastQC (version 0.11.2)