FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2573.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2573.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2352465
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAC12250.027.816824145
GTATAAG13100.026.0174471
GCCCACC11750.025.298323145
TGCCGTG7100.024.50748145
GAGACCC14800.023.513935145
CACGAGC16800.023.303988145
GTGTATA15150.022.9755921
CTGTACG15200.022.4172129
GTATATA16250.022.3128341
GTCTTAG6700.021.646781
GTATTAG8000.019.9420951
AGACCGC11400.018.443312145
CTCCGAC14550.018.436754145
CAGTGCA9250.018.0265839
GCTGATG37450.017.810732145
CCTTAAC16450.017.6328663
ATCTCCC13400.017.313742145
GTATACG6303.6379788E-1117.2658821
TACGCTA3002.4143656E-416.9205675
ATAACGT11600.016.878894
Produced by FastQC (version 0.11.2)