FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2571.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2571.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3115797
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC14550.030.395117145
TGCCGTG33600.027.403212145
CGCTACC9400.026.223343145
GTATAAG15850.024.7101751
CCACGAC15100.023.526436145
CGCTATG20400.023.100437145
CTGCTTT34950.021.573626145
GTGTATA18500.019.6024131
CGTCTTG45000.019.333288145
GTATAGG7650.018.9618131
CTATACT11550.018.2086814
CCGTGCA5651.4370016E-1017.9639849
CGAGACC22000.017.795412145
GTATTAG11550.017.5827711
ATAATAC24250.016.7470663
GTTAAGG19200.016.6212141
CTCCGAC17550.016.524178145
TATACTG21300.016.0022375
CTGTACG18850.015.7686879
TATAAGA26400.015.6583992
Produced by FastQC (version 0.11.2)