FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2570.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2570.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506839
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC5800.039.99828145
TGCCGTG5400.025.508162145
GTGTATA10150.024.2951491
TACACCG3652.1827873E-1023.8440845
TCCTATG10750.022.929247145
GTATAAG8400.022.4491961
CTAAGGG2953.8638245E-722.1265033
GTATTAG6500.021.200541
GTATAGG3901.2152668E-820.4566631
TATACCG4351.9572326E-920.0071055
TACACTG8400.018.9948435
TATATAC10700.018.9787043
TTATACT13050.018.8956014
CCCCTAG20250.018.2663541
ACCGCGT2400.001060355918.124228
AGACGGC13650.018.05783145
ACAGTGC6451.8189894E-1217.9837238
CCTAGTG18600.017.936483
TACCCCA5401.5079422E-917.4599065
CCCTAGT20550.017.2938212
Produced by FastQC (version 0.11.2)