FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2569.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2569.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1683684
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC32110.1907127465723972No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG23730.14094093666032342No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG10150.027.149721
GCGTCAG6600.026.37031
CCTAGTG39750.023.1673133
TGCCGTG8500.023.029076145
CTAGTGA49250.021.9376014
CCCCTAG45100.021.5464651
TAGTGAC48500.021.080775
AGTGACC49250.021.0542076
CACAACG11750.020.9821573
CCCTAGT44850.020.694582
CAACGTT13950.019.7523375
ATAATAC14000.019.1638493
CGTTATG13100.018.821551
ACAATGC20050.018.802728
CCACGAC9450.018.41243145
GCCCACC9100.018.323908145
ACAACGT13500.018.2622494
TGCAAGC9350.017.8371455
GTCCTAA4908.589268E-917.759591
GTGTATA9900.017.58021
Produced by FastQC (version 0.11.2)