FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2568.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2568.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913811
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC61960.21264248092961419No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG45500.15615288706096586No Hit
CTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTTGACCTCTATTT31120.10680171088653313No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAC16200.024.614141145
TGCCGTG11950.024.267729145
CACAACG22600.024.069183
GTATAAG12700.023.9874381
CAACGTT24000.023.5717535
ACAACGT23400.022.9363524
CGTTATG26450.022.7609671
CAATGCG34400.021.9178039
GTGTATA17150.021.1467761
ACAATGC40200.019.8375828
ACGTTTC48450.019.7519387
GCCCACC15100.019.685387145
CACGAGC33650.019.606197145
CCTGTGA21350.019.3635713
CCTAGTG47700.019.1584633
TTATGAC36900.019.0657463
TATGACA38050.019.0613574
GACAATG46050.019.0495767
TAGTGAC51950.018.8476285
CCCCTAG50650.018.7598841
Produced by FastQC (version 0.11.2)