FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2567.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2567.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20356
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC380.18667714678718805No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG340.1670269208095893No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCCGAT100.0070402618145.07
TCAGACG100.0070402618145.09
AATTCCT100.0070402618145.05
TACTCTA100.0070402618145.07
ATACTCT203.81127E-4108.756
AATACTC203.81127E-4108.755
CTTTTCC350.00351651662.1428579
AAGGGGG1801.1767606E-610.472221140-144
AGGGGGG2108.415942E-610.357142145
AAAGGGG2004.6569603E-58.700001140-144
TCTGCTT2907.515666E-67.5140-144
GGGGGGG59950.06.8932443145
CTGCTTG2803.0286302E-46.732143140-144
CTTCTGC3253.7196034E-56.692308140-144
GCCGTCT3551.2610664E-46.12676135-139
TGCTTGA2850.00272062286.105263140-144
CGTCTTC3702.2217543E-45.8783784135-139
TATGCCG4001.0189277E-45.8130-134
TCTTCTG3750.00167864155.4133334140-144
TCTCGTA4454.6780866E-45.2134833125-129
Produced by FastQC (version 0.11.2)