FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2566.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2566.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1833646
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC20350.11098107268251343No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7400.031.35322145
GTATAAG10450.024.9864231
TGCCGTG6750.023.631039145
GTATAGA8550.022.9042221
CCACGAC8900.022.810349145
GCAGCGT4358.367351E-1121.6756461
GTATATG8950.021.0701771
CGTCTTG12100.019.773907145
CTGCTTT18700.019.773905145
GTGTATA11550.019.4669381
CACGAGC20550.019.05223145
GTATAAT14550.018.4440651
GTACTAT5901.2732926E-1118.439841
TTAATAC8000.018.1315213
GCGTCAG4857.554263E-917.9450874
GTATATA13400.017.8618761
ACAGTGC6503.6379788E-1217.8457588
CCCCTAG30750.017.6902541
TATACCG4557.116432E-817.5347355
GTATAAA14950.017.4654271
Produced by FastQC (version 0.11.2)