FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2565.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2565.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2105097
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC23770.1129164119278114No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG22390.10636089453360106No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10550.032.98563145
GTATAAG11850.025.7070751
CGCTATG13450.022.639301145
GTATAGG6200.021.0572931
GTATATG11550.019.467111
CCTAGTG27650.019.4100823
GTGTATA12950.018.4827211
CGCTACC7500.018.366583145
CCCCTAG30900.018.3087141
GTATATA14550.017.9457341
GTCCTAG11750.017.9011881
CCCTAGT30700.017.7179572
GTATAAA17150.016.9167791
TAACGTT13550.016.593345
TGCCGTG20600.016.541187145
ACCGTGC4451.0893345E-616.292068
TAAGCCT50250.016.020944
ACCGTGT5453.2034222E-815.9632298
CGAGACC15050.015.896938145
CGGTGTG10500.015.8872965
Produced by FastQC (version 0.11.2)