FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2564.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2564.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1411265
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGGCAC3900.033.461987145
GCCCACC6600.029.65949145
GTATAAG9300.026.5179441
GTGTATA8800.023.9033761
GCGTCAG4205.2750693E-1122.451121
TGCCGTG8550.022.04708145
CTACGCT2404.3545388E-521.1521134
GTATAGG4601.7644197E-1020.4988481
GTATGGG5700.020.356923
CTATACT5002.5465852E-1120.306034
CCCACGC3651.3609497E-719.863281145
CCACGAC7450.019.463348145
GACTGTG5657.2759576E-1219.2466817
GGGTATG6301.8189894E-1218.421431
GTATAGA6301.8189894E-1218.421431
TATAAGA13000.018.4093112
TACCCCA5701.6189006E-1017.8123055
CGTCTTG12850.017.490507145
TAGGACA7500.017.4051674
TACACTG7100.017.3643115
Produced by FastQC (version 0.11.2)