FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2563.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2563.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1650728
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG7950.026.447805145
GTGTATA10450.024.2908171
CCACGAC8150.024.019554145
GCCCACC7850.024.013887145
GTATAAG9250.022.7377051
CGTCTTG12850.020.312256145
TTATACT15350.019.3704414
GTATAGG4253.2625394E-818.771291
CACGAGC9400.018.511524145
CTGTACG8750.018.2277899
ATACCCC6001.8189894E-1118.1302624
GTATTAG6052.0008883E-1117.9815141
TCCTATG12600.017.838156145
TACCCCA6253.274181E-1117.405055
CCCCTAG18800.017.3598121
GTTAAGG9200.017.343041
GTATATA11300.017.3290861
GTATATG8000.017.2247921
GTTATAG6354.0017767E-1117.1319941
TATAAGA16200.017.0116252
Produced by FastQC (version 0.11.2)