FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2560.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2560.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences754254
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC3850.037.665573145
CCACGAC3300.032.95738145
CCGTACA950.004727644430.5248939
GCGTCAG2003.253881E-728.9986464
CAGCGTC2054.0385748E-728.291362
TGCCGTG3950.027.534014145
GTATAAG5100.027.0085451
GCAGTGC1409.995026E-425.8916518
ACCCCGT1409.995026E-425.8916518
ACTGGCC1701.0312901E-425.5870428
GCAGCGT2051.2901839E-524.7549421
GTGTATA5750.022.6945951
GTCCATA2252.6513817E-522.5545041
CTGTACG3554.123649E-922.4637419
GACTGAG1952.6147426E-422.3066527
CGTCTTG7500.022.235243145
ACACATA7550.022.087992145
CCCATAC2653.7794052E-621.8857731
ACCGTAC2003.1030382E-421.7489858
AAGGCAG2353.7089572E-521.5947385
Produced by FastQC (version 0.11.2)