FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1396089
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC21410.15335698512057613No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG17150.12284317117318452No Hit
CTTACACACTTACACACTTACACACTTACACACTTACACACTTACACACT14210.10178434182921002No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTG8700.026.6702845
TGCCGTG15550.026.576313145
GTCGGTG9550.023.5372243
TCGGTGT11350.021.7210064
TGTCGGT10800.021.4859352
GTATAAG8250.021.097551
CCTAGTG30450.020.9552233
GCCCACC7500.020.300571145
ACCTTAC2205.905275E-419.7725777
CCCTGTG10350.019.6175922
TAGTGAC39450.019.2991945
CCCCTAG34200.019.2970091
CCCTAGT34700.019.016912
CTAGTGA40150.018.6015264
CAATGCG15300.018.4802519
CGTTATG11050.018.3768231
AGTGACC40250.018.3751646
GTGTAAC5251.036824E-917.958031
GCGAATG13400.017.85498145
TAAGCCT48300.017.7146194
Produced by FastQC (version 0.11.2)