FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2557.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2557.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1051716
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG3500.031.071714145
GGCCTGC950.00472279730.5324046
GAGCCCC6000.022.958542145
CTGTACG3304.6900823E-821.9698989
GCCCACC5101.8189894E-1221.323725145
GTGCTAC1750.003653622520.71941
CACGAGC7450.020.436428145
CCACGAC4652.055458E-1020.269005145
CCCTGTG4051.8819264E-819.6915389
ACCGAGC1850.005046779319.5947748
CTAGATG10400.019.51941145
GCGTCAG3351.3114368E-619.4824221
GTGTATA6700.018.4000641
GTATTAT8950.017.825631
CATAGGG2050.00911623717.6864512
CCCACGC3753.862884E-617.400162145
TAGGACA7100.017.3626164
GTATAAG4608.0681275E-817.3412381
CTATACT4608.072493E-817.3404144
TATAGTG4205.9316517E-717.2653475
Produced by FastQC (version 0.11.2)