FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1441890
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC17330.12018947353820333No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG14460.10028504254832199No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG4350.028.333265145
AGACCGC7100.026.549232145
TATACAG8350.022.583415
CCACGAC7950.021.886742145
TAAAGGT7600.020.99484
GTGTATA8600.020.2416361
GAGCCCC9050.020.027576145
ACCGTGC1950.00684208418.5896998
GTATAAG7050.018.5189441
GACAGGC2751.2427055E-418.45457
GCTTTAG3151.6778746E-518.420961
ACCCCGT3252.2041122E-517.8461118
CACGAGC11150.017.556013145
ACAGGCC2901.8661264E-417.4999588
ACCCTAG2500.001389616617.4078061
CTACGCT2500.001390262917.4065974
GCTACAC3352.8503433E-517.323
TAGAACC8250.016.70334
TCCTAAG3052.734194E-416.646232
CTACCCT3052.7349178E-416.6456554
Produced by FastQC (version 0.11.2)