FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1842054
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG8900.029.3320661
GCCCACC9300.027.284807145
CCACGAC9100.025.494375145
TGCCGTG10600.024.622517145
CCCACGC6000.022.958218145
TATACTG12350.018.787885
GTATAGA9550.018.223761
TATAAGA16800.018.1273692
CTGTACG8200.017.6823589
GTATATA12000.017.524551
CCTGTAC3753.8616E-617.4022733
GTATAAT14550.016.9451751
TTATACT16700.016.933354
CAATGCG13350.016.8346639
CTGCTTT18200.016.730684145
TACTGCG3052.7440322E-416.638817
ACAGTGC7850.016.6236698
GTGTATA10500.016.5749441
CTATACT7051.0913936E-1116.456054
TAATACT11950.016.3828954
Produced by FastQC (version 0.11.2)