FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1407361
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTG5750.031.520973145
CTGTACG6250.022.0394679
CCCACGC6950.021.905945145
GAGCCCC9050.021.629309145
GTGTATA8550.021.211881
GCTATAG3106.21083E-721.0613461
GTATAAG7650.020.862511
GTCCTAG8350.020.8511521
ACAGTGC6750.020.4069128
GCAGCGT2908.209623E-620.0123141
GTATATG7600.019.0906931
GTATTGG7250.019.0116961
TATACAG6800.018.1342265
GTATAGG4054.0364648E-717.9122541
TCTTAGG2450.001212823717.764772
CTACACG4104.5959132E-717.6919274
CACGAGC15100.017.284348145
GTACTAC4205.909369E-717.2725321
CCTAGAG10650.017.0274413
TAATACT8550.016.9676954
Produced by FastQC (version 0.11.2)