FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2848114
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC33290.11688436628590007No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACGAC15250.023.770805145
CCTAGTG59400.023.3150543
CCCCTAG65950.021.9897331
GCCCACC15200.021.464098145
TAGTGAC78400.021.4575
CTCCGAC16600.021.400885145
CCCTAGT64400.021.3926622
ACAACGT19400.021.3044474
CTAGTGA80300.020.4978084
CACAACG20250.020.4098263
CAATGCG31450.020.2857259
AGTGACC83150.020.144056
GTATTAA11950.019.417211
GTGTATA18200.019.1238171
CGTTATG22700.018.5270751
ATAATAC21200.018.4691723
GTATAAG15350.018.4230251
CCCTGTG22600.017.9670472
CAACGTT23500.017.8960535
TACACCG7300.017.8791455
Produced by FastQC (version 0.11.2)