FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2549.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2549.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1167547
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC21870.18731579970656428No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG16340.13995153942410885No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGTG17150.027.4864083
CCCCTAG20500.027.2399041
CACAACG8800.027.1956843
ACAACGT8850.026.2225824
CAACGTT9700.025.4200215
CCCTAGT19800.024.5402152
CAATGCG15500.024.3226099
GCCCACC6300.024.165659145
TAGTGAC22750.023.9082955
CGTTATG11900.023.7676581
CTAGTGA23350.023.6045364
AGTGACC24850.022.763396
GTAACGC1952.6097856E-422.3144073
CTGTGAC9150.022.1924724
ACAATGC17350.022.1478
CCTGTGA8700.021.673193
GTATAAG5900.020.8961181
CCCTAGA4551.5279511E-1020.7205222
CTGTACG7100.020.422569
TTATGAC17250.019.7595713
Produced by FastQC (version 0.11.2)