FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2548.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2548.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2452920
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG13250.030.1068041
GCCCACC14900.022.870205145
CCACGAC12100.022.769667145
GTGTATA14700.022.203071
GGTTAAG7650.019.9102041
TATAAGA23450.017.6284642
GAGCCCC16000.017.21956145
CCCACGC13050.016.667452145
GTATAAA19800.016.4840971
CCCCTAG36700.016.403251
GTATAGG6709.276846E-1116.2380661
CTGCTTT27850.016.140797145
TACTGTG11250.016.1105587
CTAGGAC16000.015.86432653
CTGTACG7352.1827873E-1115.7817699
TGTATAA19800.015.7501852
TACACAG11100.015.680575
ACTAGGG38450.015.65542
TATACCG6056.8193913E-915.5833765
TAAGAGA30750.015.5658344
Produced by FastQC (version 0.11.2)