FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2546.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2546.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1434527
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC7950.033.74471145
GTGTATA8500.030.7146471
GTATAAG8550.024.5950893
ACTGTGC4800.022.6556078
CTCCGAC8150.021.35132145
TGTATAA11550.021.345852
GTATATA8900.021.1858221
CTACGCG2104.3162177E-420.71379
CCACGAC9200.020.490692145
GTATACG3901.2165401E-820.4545541
GTATAAC5052.910383E-1120.1047481
CTGTGCG4102.1693268E-819.4506689
CTTAGAC3751.8060928E-719.3368283
TCCTGAC7750.018.711102145
ACCGTGC3101.467861E-518.7091468
CCCTTAG3201.9241505E-518.1301731
CTGCACG2801.4269384E-418.1244859
CTTATAA13850.017.79919145
TATAAGA14300.017.7479634
GTACAAG7050.017.4872591
Produced by FastQC (version 0.11.2)