FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2545.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2545.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1519364
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC15300.10070002974928982No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC6350.034.250618145
TGCCGTG4900.029.590668145
CTGTACG7300.024.8277879
GTATAAG8200.022.995223
ACTGGGC3203.3898687E-822.6553558
GTGTATA10050.022.3718721
TGTATAA12950.020.1609422
GTAAGAC5052.910383E-1120.1054943
CCTAATA6451.8189894E-1217.9902972
GTATTAG6152.5465852E-1117.6897961
TAGGACA10250.017.6886314
CCTTAAC9150.017.4373193
CTAATAC7150.017.2433223
CGTCTTG12100.016.776197145
CATGATG27350.016.699524145
CTTAACT10000.016.680384
GTATAGG4358.5520514E-716.6731411
CTAAGGC2650.002053993316.420393
ACTGTGC5302.2711902E-816.4144468
GTATAAT12600.016.117371
Produced by FastQC (version 0.11.2)