FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2165921
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA12800.030.593011
GTATAAG14050.028.3873461
GCCCACC9900.026.363035145
CACGAGC15700.020.77978145
CCCACGC7400.020.573856145
CTAGCAC3951.411172E-820.193613
CTCCGAC12750.019.332893145
CCACGAC9900.019.03997145
TGTATAA17600.018.9528062
GTATAGG5951.4551915E-1118.2815361
ATCTCCC13100.018.262943145
TATAAGA20900.018.0420322
CTGCTTT22950.018.006124145
GTATAGA11700.017.3544391
TAAGAGA27250.017.0310674
CTGCACG3956.3429015E-616.5186129
ATAAGAG26000.016.4549673
CCCCTAG24750.016.1148361
GTATATA15350.016.0623461
ATAATAC15400.016.0094553
Produced by FastQC (version 0.11.2)