FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2541.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2541.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1757648
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC28030.15947447953173788No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG21350.12146914513031051No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC8450.030.886208145
GTATAAG13350.029.8862951
GTGTATA12650.029.8197151
TGCCGTG5750.026.477087145
CCACGAC7350.023.67242145
TAGGACA9000.023.369384
TATAAGA19150.022.7240922
TGTATAA17150.021.9909152
GTATATA12250.021.318521
CTAGGAC7500.019.3401783
CTGTACG10900.019.2881369
TTACGCG1900.005891133619.0781029
GTTAAGG14950.018.4388181
GCGTCAG6750.018.2698841
CTCCGAC11200.018.124197145
CAGGACT5501.9244908E-917.1424314
TAGGAGT5602.4447218E-916.8363174
ATCCCGC3453.670143E-516.8175476
CCCCTAG18700.016.6808241
TAAGCCT64400.016.6673894
Produced by FastQC (version 0.11.2)