FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2540.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2540.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2086345
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCACC10300.032.378777145
CCCACGC9100.026.291317145
GTATAAG12250.026.047791
GTGTATA14050.024.7753161
GGACCGT3304.6864443E-821.9746
GTATTAG7700.019.7780231
GTATATG10250.018.3951681
CTATACT7500.018.3702644
CTCCGAC12800.018.125076145
GTATATA13750.017.9320741
ACCGTGC4104.6229434E-717.6829998
ATCTCCC11800.016.589052145
TATAAGA19800.016.4808962
TAAGAGA25550.016.4611514
TAATACT12450.016.3084164
TACACAG10800.016.1142675
GTATAGA10550.015.8099191
CCACGAC11600.015.625065145
GTATAAT18150.015.5826851
TGTATAA17750.015.5245412
Produced by FastQC (version 0.11.2)