FastQCFastQC Report
Mon 21 Mar 2016
H7NV2AFXX_n01_qg2539.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7NV2AFXX_n01_qg2539.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1072030
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCTAA1801.2886994E-732.233171
TGCCGTG6250.029.000381145
GTATAAG5700.025.447241
GGCCGTA1450.001226406325.0003327
GCTTTAG2401.5935402E-624.1748811
GCCCACC5100.024.166985145
GTGTATA6400.023.7971481
ACAGTGC4551.5279511E-1020.714568
TCTATAC5002.5465852E-1120.3050043
GTACTAG5204.3655746E-1119.5258661
GTCTTAA4202.8461727E-818.9945491
TCTAGAC7650.018.958923
TATACTT7750.018.7151625
CTATAGT3151.6787926E-518.4189551
GTATAGG3151.6787926E-518.4189551
TTAGTAC3151.6801423E-518.4172363
CCTAGTG9550.018.2243863
GTATTAA5209.113137E-1018.131161
TATACCG2801.4230039E-418.1303145
CGTCTTG10100.017.94578145
Produced by FastQC (version 0.11.2)